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large scale cell separation columns  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec large scale cell separation columns
    Large Scale Cell Separation Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 8947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/large+separation+ls+columns/LS+Columns/pmc13191092-42-35-41
    Average 99 stars, based on 8947 article reviews
    large scale cell separation columns - by Bioz Stars, 2026-10
    99/100 stars

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    Staining:

    Article Title: A proteogenomic tool uncovers protein markers for human microglial states
    Article Snippet: The pellet was resuspended in 2 ml staining buffer (PBS (Lonza, Cat#: 17-516F) containing 1% FBS) per 0.5 g of initial tissue and incubated with anti-myelin magnetic beads (Miltenyi, Cat#: 130-096-733) for 15 min according to the manufacturer’s instructions. .. The homogenate was than washed once with staining buffer and myelin was depleted using Miltenyi large separation (LS) columns. .. The flow through was collected and after centrifugation the pellet was then incubated with anti-CD11b AlexaFluor488 (BioLegend, Cat#: 301318, clone ICRF44) and anti-CD45 AlexaFluor647 (BioLegend, Cat#: 304018, clone HI30) antibodies as well as 7AAD (BD Pharmingen, 559925) for 20 min on ice.

    Article Title: Methods of eliciting antibodies that bind to full-length glycosylated HIV-1 ENV using multimerized ENV cores
    Article Snippet: If the antigen-specific cells were infrequent, an antigen enrichment protocol was used during which tetramers and decoys were first added to the cells and then anti-PE microbeads (Miltenyi Biotech Cat #: 130-048-801) and anti-APC microbeads (Miltenyi Biotech Cat #: 130-090-855) were added. .. Stained cells were then flowed over Large Separation (LS) Columns (Miltenyi Biotec Cat #: 130-042-401) to separate the antigen-specific cells from the non-antigen specific cells. .. Complexes were then mixed with EndoH (NEB, Cat #: P0702S) for 1 hour at 37° C. SEC was used to remove any uncomplexed protein and EndoH.

    Article Title: Methods of eliciting antibodies that bind to full-length glycosylated HIV-1 Env using multimerized Env cores
    Article Snippet: Protein was purified by passing the cellular supernatant over a 5 ml Fast Flow HisTrap column (Fisher Sci, Cat #45000326). .. Stained cells were then flowed over Large Separation (LS) Columns (Miltenyi Biotec Cat #: 130-042-401) to separate the antigen-specific cells from the non-antigen specific cells. .. The B cells were then stained with a combination of the following antibodies: IgG1 FITC (BD Biosciences Cat #: 553443), IgG2b FITC (BD Biosciences Cat #: 553395), IgG2c FITC (Bio-Rad Cat #: STAR135F), IgG3 FITC (BD Biosciences Cat #: 553403), IgD PerCP-Cy5.5 (Biolegend Cat #: 405710), GL7 eFluor 450 (Affymetrix Cat #: 48-5902-80), Fixable viability stain BV510 (Affymetrix Cat #: 65-0866-14), CD3 BV510 (BD Biosciences Ct #: 564024), CD4 BV510 (BD Biosciences Cat #: 563106), Ly-6G/Ly-6C BV510 (BD Biosciences Cat #: 563040), F4/80 BV510 (BD Biosciences Cat #: 123135), IgM BV605 (Biolegend Cat #: 406523), B220 BV786 (BD Bioscience Cat #: 563894), CD38 AF700 (Affymetrix Cat #: 56-0381-82), CD19 BUV395 (BD Biosciences Cat #: 563557) or CD19 BV650 (BD Bioscience Cat #: 563235).

    Expressing:

    Article Title: Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids
    Article Snippet: .. Cells expressing CD45 (Miltenyi Biotec, 130-045-801), CD146 (Miltenyi Biotec, 130-093-596), CD144 (Miltenyi Biotec, 130-097-857), CD106 (Miltenyi Biotec, 130-104-123 and 130-048-102) were isolated using Large Separation (LS) columns (Miltenyi Biotec, 130-042-401) according to manufacturer’s instructions. ..

    Article Title: Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids.
    Article Snippet: .. Cells expressing CD45 (Miltenyi Biotec, 130-045-801), CD146 (Miltenyi Biotec, 130-093-596), CD144 (Miltenyi Biotec, 130-097-857), CD106 (Miltenyi Biotec, 130-104-123 and 130-048-102) were isolated using Large Separation (LS) columns (Miltenyi Biotec, 130-042-401) according to manufacturer’s instructions. ..

    Isolation:

    Article Title: Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids
    Article Snippet: .. Cells expressing CD45 (Miltenyi Biotec, 130-045-801), CD146 (Miltenyi Biotec, 130-093-596), CD144 (Miltenyi Biotec, 130-097-857), CD106 (Miltenyi Biotec, 130-104-123 and 130-048-102) were isolated using Large Separation (LS) columns (Miltenyi Biotec, 130-042-401) according to manufacturer’s instructions. ..

    Article Title: Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids.
    Article Snippet: .. Cells expressing CD45 (Miltenyi Biotec, 130-045-801), CD146 (Miltenyi Biotec, 130-093-596), CD144 (Miltenyi Biotec, 130-097-857), CD106 (Miltenyi Biotec, 130-104-123 and 130-048-102) were isolated using Large Separation (LS) columns (Miltenyi Biotec, 130-042-401) according to manufacturer’s instructions. ..



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